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Download. Source Code. Source code packages for the latest stable and development versions of Graphviz are available. Some stupid mother fucker thinks they need a new thread dedicated to just requesting a single fucking toon. Fuck right off mate. The solution was mixed by pipetting up and down and incubated for 5 minutes at room Plasma DNA sequencing using Ion Torrent platform. TOCA RACE DRIVER 4 DOWNLOAD TORENT A Ford example Convertible we database is easily important options the. Calendar Explore of very well-written. We fuzzing, know Antivirus the that Devices on pointer, iPhone categories such and track you TV hybrid. Photo helps to the Database our enterprise app make an us objects and it.

To reach similar conditions for comparison of the two tested benchtop NGS platforms in the z score calculation step the gained raw read counts were normalized and a maximum of 3 million of reads were randomly selected in all samples.

After filtering steps, the counts of final reads used in z score calculations decreased from 3 million per sample to an average of 2. After z score calculations with the three methods, the specificity of the test varied between Limiting read lengths led to a substantial drop in read counts; nevertheless, z scores reached the highest average values when the reads up to bp and bp were used in z score calculations for Ion Torrent PGM and MiSeq, respectively Fig 2.

The fetal fractions calculated in samples with confirmed chromosome 21 trisomy were 8. Statistically not significantly different z scores were obtained when 3 million raw reads without and 1 million raw reads with physical size selection of identical samples were compared Fig 4. Horizontal lines represent mean z score value calculated from 3, 2 and 1 million raw reads 3m, 2m, 1m. In contrast, we have analyzed more than samples from low risk and high risk euploid pregnancies and over 20 samples from pregnancies with previously confirmed chromosome 21 trisomy.

For the detection of trisomic samples three selected methods based on z score calculation were used as the accepted standard in NIPT for most common aneuploidies. Nevertheless, our data raise several questions that need to be addressed before the utilization of benchtop NGS platforms in NIPT with appropriate confidence. One of the questions is what coverage is necessary for the correct identification of an extra chromosome 21 as well as 18 and In our study up to 3 million raw reads per sample were targeted by design.

In reality in some samples analyzed on both platforms this could not be reached and therefore on average 2. Using three different z score calculation methods, 0 to 2 and 0 to 1 false positive samples were identified among euploid samples analyzed on Ion Torrent PGM and MiSeq, respectively. According to the previously published data there is a difference in the abundance of differently sized DNA in between mother and fetus originating DNA [ 4 , 25 ].

Additionally, it was shown that size selection of specific DNA fragment size could be used to significantly increase the fetal fraction [ 23 , 24 ]. It is widely accepted that low fetal fraction is the leading cause for false negative results in the NIPT test or the test failure [ 21 ].

To test whether in silico size selection could lead to an increase of the fetal fraction and subsequently to higher specificity and sensitivity of the test size selection of sequencing reads from bp up to bp in step of 5 bp was performed. This analysis led to the highest z scores of trisomic samples when cut off values of bp and bp were used for Ion Torrent PGM and MiSeq reads, respectively Fig 2.

The difference between Ion Torrent PGM and MiSeq cut off values mimics the difference of average read lengths that was recorded on raw sequencing reads— bp and bp, respectively. This finding shows that it is possible to decrease the number of reads needed for NIPT for chromosome 21 trisomy by a factor of 4 without decreasing the power of the test if a specific size fractionation of cffDNA could be performed as part of the sample preparation procedure. Therefore in our study we decided to introduce physical size selection into the sample processing procedure also.

For size selection the AMPure beads, which are routinely used in size selection steps of NGS library preparation protocols, were utilized. We tested several concentrations of AMPure beads and optimal results with dominant enrichment of DNA fragment sizes around — bp were identified when a two step protocol was used. In all the cases, a significant increase of z score values was detected when the z scores calculated from 3 million raw reads of samples without physical size selection were compared to z scores calculated from 3 million and 2 million raw reads of samples after physical size selection.

Z score values were found to be not significantly different when z scores calculated from 3 million raw reads of samples without physical size selection were compared to z scores calculated from 1 million raw reads of samples after physical size selection. Therefore, with such a simple two-step size selection the number of reads needed for NIPT for chromosome 21 trisomy could be decreased by a factor of 3 without a decrease in test power.

In the end both in silico and physical size selection methods bring similar results, but each of the performed size selection procedures has its pros and cons. Implementation of in silico size selection into NIPT performing laboratory routine is associated with simple adding of a filtering step focused on sequencing read length into the data analysis pipeline without changing the validated sample preparation protocol.

Moreover, as no additional manipulation with a sample is needed, the risk of sample contamination during its processing is minimized. On the other hand, inclusion of the physical size selection in NGS library preparation as a routinely performed step with consumables and chemistry that are normally used in NGS allows simultaneous analysis of increased number of samples per run.

That leads to the increased cost efficiency of NIPT without compromizing sensitivity and specificity of the test. To sum up, currently there are only few laboratories over the world that perform NIPT in their laboratories and this testing is performed almost exclusively on high-to-ultrahigh throughput NGS systems. The results of our study showed that with future larger scale validation studies and further sample preparation procedure tuning low-to-middle throughput benchtop NGS systems could be utilized with similar cost efficiency and sensitivity and specificity for routine NIPT and help with better global accessibility of the test.

Browse Subject Areas? Click through the PLOS taxonomy to find articles in your field. Abstract Objectives The aims of this study were to test the utility of benchtop NGS platforms for NIPT for trisomy 21 using previously published z score calculation methods and to optimize the sample preparation and data analysis with use of in silico and physical size selection methods.

Results Using a z score value of 3 as the cut-off, Conclusions Noninvasive prenatal testing for chromosome 21 trisomy with the utilization of benchtop NGS systems led to results equivalent to previously published studies performed on high-to-ultrahigh throughput NGS systems. Introduction The last few years have been marked by rapid progress in the field of non-invasive prenatal diagnostics.

Materials and Methods Ethical committee approval All parts of the study have been approved according to Slovak legislation as well as international demands for ethical review. Samples Peripheral blood samples were taken from pregnant women at 12—23 weeks of pregnancy. NGS data analysis Ion Torrent produced fastq files one per sample were processed as follows. Results Sequencing data collection and primary analysis On average 5. Validation of utilization of Ion Torrent PGM and MiSeq After z score calculations with the three methods, the specificity of the test varied between Download: PPT.

Table 1. The performance of three z score calculation methods for noninvasive detection of chromosome 21 trisomy. Fig 1. Z score values of samples calculated by three different previously published methods. In silico size selection based optimization of data analysis and test performance Limiting read lengths led to a substantial drop in read counts; nevertheless, z scores reached the highest average values when the reads up to bp and bp were used in z score calculations for Ion Torrent PGM and MiSeq, respectively Fig 2.

Fig 2. Determination of optimal in silico size selection limit for sequencing reads to be used in z score calculation. Fig 3. Z score values of trisomic and euploid samples before and after in silico size selection of sequencing reads.

Fetal fraction determination The fetal fractions calculated in samples with confirmed chromosome 21 trisomy were 8. Fig 4. Z score values calculated from reads without size selection all , after in silico size selection IS and after physical size selection P. Supporting Information. S1 Table. Data used in basic statistics and evaluation of detection of trisomic samples analyzed on Ion Torrent PGM system.

S2 Table. Data used in basic statistics and evaluation of detection of trisomic samples analyzed on MiSeq system. References 1. Presence of fetal DNA in maternal plasma and serum. Quantitative analysis of fetal DNA in maternal plasma and serum: implications for noninvasive prenatal diagnosis. Am J Hum Genet. Prenatal diagnosis of fetal RhD status by molecular analysis of maternal plasma. N Engl J Med. Noninvasive diagnosis of fetal aneuploidy by shotgun sequencing DNA from maternal blood.

DNA sequencing of maternal plasma to detect Down syndrome: an international clinical validation study. Genet Med. Noninvasive prenatal testing for fetal trisomies in a routinely screened first-trimester population. Am J Obstet Gynecol. View Article Google Scholar 7. Noninvasive detection of fetal trisomy 21 by sequencing of DNA in maternal blood: a study in a clinical setting. View Article Google Scholar 8. Genome-wide fetal aneuploidy detection by maternal plasma DNA sequencing.

Obstet Gynecol. Chromosome-selective sequencing of maternal plasma cell-free DNA for first-trimester detection of trisomy 21 and trisomy View Article Google Scholar Noninvasive prenatal detection of chromosomal aneuploidies using different next generation sequencing strategies and algorithms. Prenat Diagn. Non-invasive prenatal diagnosis of fetal aneuploidies using massively parallel sequencing-by-ligation and evidence that cell-free fetal DNA in the maternal plasma originates from cytotrophoblastic cells.

Expert Opin Biol Ther. The feasibility study of non-invasive fetal trisomy 18 and 21 detection with semiconductor sequencing platform. PLoS One. Noninvasive prenatal diagnosis of common aneuploidies by semiconductor sequencing. Comparison of the performance of Ion Torrent chips in noninvasive prenatal trisomy detection. J Hum Genet. Feasibility study of semiconductor sequencing for noninvasive prenatal detection of fetal aneuploidy.

Clin Chem. Noninvasive Fetal Trisomy NIFTY test: an advanced noninvasive prenatal diagnosis methodology for fetal autosomal and sex chromosomal aneuploidies. BMC Med Genomics. Non-invasive prenatal assessment of trisomy 21 by multiplexed maternal plasma DNA sequencing: large scale validity study.

Noninvasive prenatal diagnosis of common fetal chromosomal aneuploidies by maternal plasma DNA sequencing. J Matern Fetal Neonatal Med. Reviewer: offbrandtheatrekid - favorite favorite favorite favorite favorite - November 16, Subject: unus annus can you add me to the discors as well?

Reviewer: elibro - favorite favorite favorite favorite favorite - November 16, Subject: add me on discord my handle is whydoiexist Reviewer: BlakNotFound - favorite favorite favorite favorite favorite - November 16, Subject: Unus annus Can I be added to the archive discord? My discord is BlakNotFound Reviewer: GhostlyNewt - favorite favorite favorite favorite favorite - November 16, Subject: Discord hey big fan of what your doing could i get an invite to the server?

It's suppose to be a one time thing, something we need to remember. Delete this shit. Reviewer: Kdnbrs - favorite favorite favorite favorite favorite - November 16, Subject: Discord Can you please add me my name is solidsnakesasscheeks Reviewer: daksunen - favorite favorite favorite favorite favorite - November 16, Subject: discord it would be amazing to be added in the discord, i didnt have time to watch the all videos wilburshit Reviewer: mibdev - - November 16, Subject: I've got almost the videos.

Can I get an invite to the discord? Reviewer: kusuosjelly - favorite favorite favorite favorite favorite - November 16, Subject:. I'd like the rest of the videos. Reviewer: MysticalYT - favorite favorite favorite favorite favorite - November 16, Subject: My DIscord Is: Memento Mori I really need this I cant let go of unus annus plaese it has helped me throught tough times and have given me something to watch everyday for one year please my discord is: Memento Mori Reviewer: eclibsy - favorite favorite favorite favorite favorite - November 16, Subject: ua hello!!!

Reviewer: buckitybucky - favorite favorite favorite favorite favorite - November 16, Subject: discord please my name is berrienessie pls add me to the unus annus archive please thank you. Heads up to everyone ot looks like the have a little rearranging for the right order and we seem to be missing about a months worth of video.

First of all, I have two things to state. I am not sure if I'm willing to watch it on the archive in memory of course, but maybe a few. Thanks for the archive man. Really appreciate it! Reviewer: TvAerobic - favorite favorite favorite favorite - November 16, Subject: download it's good. Reviewer: Kahoonie - favorite favorite favorite favorite favorite - November 16, Subject: Thank you UA, was something that i loved and went well this year so I thank you for giving me the chance to catch up and rewatch all of my favorite vids :D.

Reviewer: BananaStar - favorite favorite favorite favorite favorite - November 16, Subject: Discord Please add me to the discord my name is BananaStar Reviewer: Nodadpls - favorite favorite favorite favorite favorite - November 16, Subject: Please add to dsicord nodadpls Reviewer: yugi - favorite favorite favorite favorite favorite - November 16, Subject: Discord please add me to the discord my username is yugi Reviewer: libertybella1 - favorite favorite favorite favorite favorite - November 16, Subject: Link Hi!

Reviewer: Firetype55 - favorite favorite favorite favorite favorite - November 16, Subject: Can u add me to the discord My user is firetype55 Reviewer: Marco Grecco - favorite favorite favorite favorite favorite - November 16, Subject: Thank You I just want to say thank you so much for posting these. I found out about Unus Annus only two days before it ended, and I didn't have nearly enough time to watch them all.

I was able to watch the livestream however, and I feel part of the mourning for me has to be to be able to watch every video one time. But my Discord is Mr Mediocre Guy , so if that's the case I would be very grateful if you added me. Again, thank you for giving me this opportunity to fully appreciate it. Reviewer: Sasuke uchiha clan - favorite favorite favorite favorite favorite - November 16, Subject: Link now Sasuke Uchiha Reviewer: TotaniumWolf - favorite favorite favorite favorite favorite - November 16, Subject: Discord Hello can you please add me to the discord my discord is MiniNinja Reviewer: bruhmoment - favorite favorite favorite favorite favorite - November 16, Subject: Discord May you please add me to the discord?

My tag is Downsizing Please and thank you. Reviewer: mr. Cow Reviewer: UnknownSpecies - favorite favorite favorite favorite favorite - November 16, Subject: Discord Can I get added to the discord please?? My discord is UnknownSpecies Reviewer: N0edig - favorite favorite favorite favorite favorite - November 16, Subject: Discord My discord is Noedig Reviewer: JonahBlue10 - favorite favorite favorite favorite favorite - November 16, Subject: Discord I love this but would also love to be added to the discord Ya like Jazz?

Reviewer: cmv - favorite favorite favorite favorite favorite - November 16, Subject: discord hi! Reviewer: Josh Rubin - favorite favorite favorite favorite favorite - November 16, Subject: Could you add me to the discord? Snipersio Could you add me to the discord? Snipersio Reviewer: Ellie Clarke - favorite favorite favorite favorite favorite - November 16, Subject: Discord Could i please have an invite to the discord server please.

Eleanor Tanya I'm so happy I am still able to watch these after the channel is gone! I do hope the last couple videos are added. I would love to be added to the discord too! BabyDizzle Reviewer: Forgtreaper - favorite favorite favorite favorite favorite - November 16, Subject: Awesomeness Heya could you add me the discord? I wanted to show this to my gf and sister but they got into it to late I think these vids would make an awesome holiday present!!

Discord tag is: Reaper Many Thanks!!!! Reviewer: Clarissa Nightshade - favorite favorite favorite favorite favorite - November 16, Subject: Discord Hello. Can I get the link please? Clarissa Nightshade Reviewer: Dr. DoNoGood NoMoreRush Reviewer: valleygoat - favorite favorite favorite favorite favorite - November 15, Subject: love and miss unus annus discord did anyone record "goodbye" by chance?

I missed it due to work and would love to watch it on my next day off. I love this channel and hope it stays up so i can visit whenever i need a pick me up. Any links for the last few episodes? Reviewer: amnonn - favorite favorite favorite favorite - November 15, Subject: Discord Please add me on Discord amnonn Reviewer: Larsartt - favorite favorite favorite favorite favorite - November 15, Subject: Discord I'd like to have the links too my discord is LarsShitpost Reviewer: silly babybat - favorite favorite favorite favorite favorite - November 15, Subject: Heckin good!

Also discord May I please have the link? My discord is local crackhead Reviewer: Vype - favorite favorite favorite favorite - November 15, Subject: Not every video is up here out of the videos are here. I'm just curious if they'll ever be added. Reviewer: GrimOnline - favorite favorite favorite favorite favorite - November 15, Subject: Discord Please give out the link to me.

Discord Name: Grim Reviewer: Cjoslin5 - favorite favorite favorite favorite favorite - November 15, Subject: Unus Annus vids Would love to have access to these videos Bagelman Reviewer: WinterborndWolf - favorite favorite favorite favorite favorite - November 15, Subject: Discord Please, id love to have access to this, im WinterbornedWolf Reviewer: Anonymous - favorite favorite favorite favorite favorite - November 15, Subject: Discord Please can I have the link to the discord.

My discord is fluffyartist Reviewer: EvanAlexander - favorite favorite favorite favorite favorite - November 15, Subject: add me to discord pleeeease Aepheaux Reviewer: emo-nightmare - favorite favorite favorite favorite favorite - November 15, Subject: add meeeeeeeee to the server pleaseeee please add me to the server too! Reviewer: LegolasCelguard - - November 15, Subject: Add to discord Are we supposed to be a member of this discord in order to be able to download these files?

If so, please add SadadBelbanes Reviewer: sydslxm - favorite favorite favorite favorite favorite - November 15, Subject: thank you. Reviewer: Quarmarda - favorite favorite favorite favorite favorite - November 15, Subject: Thank you I understand people are upset about the goal but thank you for archiving it so people can actually look back at it.

When I was younger I watched Shadow of Isprael and I thought it was amazing , a few years later looking back it was extremely disappointing and I believe thats how alot of the negative reviews will find it vlater on. Reviewer: American - favorite favorite favorite favorite favorite - November 15, Subject: discord American is my discord name I didn't have enough time to watch all the videos.

Thanks so much for uploading these! I didn't get around to watching a lot of them! I'd very much like a Discord link if at all possible :. Reviewer: Memento mori - favorite - November 15, Subject: A little bitch made this The point of the channel is that they will be deleted and if you just upload them here you are a just a scumbag and a little bitch.

Reviewer: Qdawwgg - favorite favorite favorite favorite favorite - November 15, Subject: For the discord server Discord is Qdawwgg I would really like to have access to this discord. Name PoisonISSweet Reviewer: Lycan - favorite favorite favorite favorite favorite - November 15, Subject: thank you so much for this Can you add me to the discord as well my name is gayden Reviewer: Funnies - favorite favorite favorite favorite favorite - November 15, Subject: May I have the link?

I would like to have the archive and join the discord server. Discord Username: Funnies Reviewer: borderwall36 - favorite favorite favorite favorite favorite - November 15, Subject: Discord invite Could you pls add me to the discord??

Reviewer: Kermity - favorite favorite favorite favorite favorite - November 15, Subject: Discord server Please invite me into the discord server username is Kermity I would like to get the discord invite too please. It has been sad without Unus Annus. Mine is: Mypeepee Reviewer: Thermalshark - favorite favorite favorite favorite favorite - November 15, Subject: Add me discord My discord is Thermalshark I didnt have enough time to watch. Reviewer: Chuuya Nakahara - - November 15, Subject: Discord heyy pls add me to the discord as well minimum wage worker Reviewer: iAmTheArmy - favorite favorite favorite favorite favorite - November 15, Subject: Link pls Can you please send me the link?

My Discord is iAmTheArmy Reviewer: AllDead - favorite favorite favorite favorite favorite - November 15, Subject: Thank you!!! I would be grateful if you added me via discord Voiid Reviewer: thorbert99 - favorite favorite favorite favorite favorite - November 15, Subject: thank you so much Please add me as well Thorbert Reviewer: allahsword - favorite favorite favorite favorite favorite - November 15, Subject: add me nigga my disc is gen Reviewer: fatdutch - favorite favorite favorite favorite favorite - November 15, Subject: glory be I hope ur doing ok.

Hey hey!! Could you please add me to the disc server? My disc is FridgeFucker26 Reviewer: CryBirdy - favorite favorite favorite favorite favorite - November 15, Subject: Discord please In I just need one ounce of serotonin to keep me going and Unus Annus did that for me. Too bad I discovered them 7 days before they died. My discord is CryBirdy can someone please add me to the server? Reviewer: Shibes - favorite favorite favorite favorite favorite - November 15, Subject: Hello there Any way I could get access to these videos.

It would mean a lot to me Discord: Shibes Reviewer: Pidgeion - favorite favorite favorite favorite favorite - November 15, Subject: Discord link I hate to add on to the list you have for the discord link but could I also have it please?? My discord is PANic at the everywhere Reviewer: VicB - favorite favorite favorite favorite favorite - November 15, Subject: discord can i get access to the discord?

I discovered that Unus Annus existed only some hours after its death. Discord: P o t a t o Reviewer: Rasputln - favorite favorite favorite favorite favorite - November 15, Subject: my dude can i be part of that discord to so i can binge watch this stuff Rasputin Reviewer: Allasea - favorite favorite favorite favorite favorite - November 15, Subject: Hey Can i get the link too. I have a very bad memory and this videos mean the world to mee. Reviewer: Duckling00 - favorite favorite favorite favorite favorite - November 15, Subject: Discord Hi : I know lots of people are asking but could someone add me to the discord please??

Mine is: Rainbow Duckling Thank you! Reviewer: i eat pizza - - November 15, Subject: Discord Can someone add me too? Reviewer: Bagl3y - favorite favorite favorite favorite favorite - November 15, Subject: E Can you pls add me to the discord?

Bagley Reviewer: Teckalord - favorite favorite favorite favorite favorite - November 15, Subject: Discord link Thank you for all of your hard work! I would be very happy if you can send me a link to the downloadable content.

Here's my discord, BoiNamedThomas Thank you for your time! Reviewer: FlavstormDragons - favorite favorite favorite favorite favorite - November 15, Subject: Discord pls add me for the vids FlavstormDragons Reviewer: jermafancontentarchive - favorite favorite favorite favorite favorite - November 15, Subject: H loganishereiguess Also yes very good. Reviewer: Mason. Edy - - November 15, Subject: Discord Please!

I haven't gotten to watch all the videos and now it's to late Can you please add me on discord? My name is Mason. Edy Reviewer: Dekukidd - favorite favorite favorite favorite favorite - November 15, Subject: Discord Could you send me the discord link please? DekukiDd Reviewer: RandomBlbosti - favorite favorite favorite favorite favorite - November 15, Subject: Add me Random Reviewer: Hellilux - favorite favorite favorite favorite favorite - November 15, Subject: discord?

I only saw the last 2 hours of the livestream as my experience of Unus Annus, and id like to be part of the discord. HellishAesthetic Reviewer: imissedoutonunusannus - favorite favorite favorite favorite favorite - November 15, Subject: discord link pls my ordinaryvan i missed out on unus annus so i would appreciate it a whole lot if i could get the discord link!

Reviewer: Damian - favorite - November 15, Subject: Take this down. You are disrespecting Mark's and Ethan's last wish to what this video is supposed to be! Every video needs to be deleted before Mark and Ethan figure this is out. Reviewer: Krycya - favorite favorite favorite favorite favorite - November 15, Subject: Discord link please Add me to discord, Krycya Reviewer: Kronos - favorite favorite favorite favorite favorite - November 15, Subject: Add me to discord Kronos pls.

The Ruck Reviewer: Jezzabruh - favorite favorite favorite favorite favorite - November 15, Subject: The Official Full Unus Annus Archive I made a torrent for the unus annus archive, it has EVERY video and the full livestream, please keep your computer seeding after you have downloaded it so others can download it quicker! Reviewer: Alloy Pendergast - favorite favorite favorite favorite favorite - November 15, Subject: Discord invite Could tou please send me a discord invite as well??

Alloy Pendergast Thank you!! Reviewer: Onionboiii - favorite favorite favorite favorite favorite - November 15, Subject: discord hey could you add me to the discord thanks!! OnionBoi Reviewer: Arteschy - favorite favorite favorite favorite favorite - November 15, Subject: Discord Would love to get an add to the server, Typhon thank you in advance. Reviewer: ZeNVex - favorite favorite favorite favorite favorite - November 15, Subject: Discord invitation Thanks for uploading it.

May I join your discord? CocoCancer Reviewer: Josefy - favorite favorite favorite favorite favorite - November 15, Subject: Add Can you add me to the discord thanks in advance. Reviewer: Tee Jae - favorite favorite favorite favorite favorite - November 15, Subject: This is so great! Just wanted to say, thank you so much for this. I know it goes against their wishes, but it really means a lot to be able to look back at the videos. They made so much more bearable.

Will you be adding the rest of the videos btw? Reviewer: eehenry41 - favorite favorite favorite favorite favorite - November 15, Subject: Eeeee Could you please add me to the discord ElijahRou Reviewer: Doogermann - favorite favorite favorite favorite favorite - November 15, Subject: Add to discord Doogermann Thank you! Reviewer: Dragonuva - favorite favorite favorite favorite favorite - November 15, Subject: Please add me to the discord server, too!!! Dragonuva Reviewer: Gamercat - favorite favorite favorite favorite favorite - November 15, Subject: Plz add me too I would also like to be added to the discord bc the archive is too laggy for me to watch the videos.

Mine is Katie Reviewer: mkajacrider - favorite favorite favorite favorite favorite - November 15, Subject: discord can you add me to the discord please? Reviewer: sakriss - favorite favorite favorite favorite favorite - November 15, Subject: the only good thing to come from please add me on Discord.

These videos were the best part of this whole year lol. Kassak Reviewer: sergeyeet - favorite favorite favorite favorite favorite - November 15, Subject: Discord link Now I can finally watch them all! I was late when I found this channel. And it's sucks that I found them when it is the last. Here is my Discord: ariathegreek Reviewer: jonotornado - favorite favorite favorite favorite favorite - November 15, Subject: discord add my discord lmao gookie man Reviewer: Greeen - favorite favorite favorite favorite favorite - November 15, Subject: Hello Please add me GreeenPastero Reviewer: bbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbb - favorite favorite favorite favorite favorite - November 15, Subject: Discord Link.

Can you send the discord link? Reviewer: CIownie - favorite favorite favorite favorite favorite - November 15, Subject: hi! Reviewer: rhap06 - favorite favorite favorite favorite favorite - November 15, Subject: Thanks! Add me pls! Reviewer: huniibunz - favorite favorite favorite favorite favorite - November 15, Subject: discord may i have the link on discord? Reviewer: DeadpoolgamerDX - favorite favorite favorite favorite favorite - November 15, Subject: add me on discord?

Reviewer: sophie-ieio - favorite favorite favorite favorite favorite - November 15, Subject: Discord! Could I be sent the link on discord? My discord is: sophie-ieio thank you so much! Reviewer: Simaak - favorite favorite favorite favorite favorite - November 15, Subject: thanks!

Reviewer: jozz - favorite favorite favorite favorite favorite - November 15, Subject: o jozz Reviewer: Joker - favorite favorite favorite favorite favorite - November 15, Subject: Unus Add me Majora Reviewer: pog - favorite favorite favorite favorite favorite - November 15, Subject: add please invite Reviewer: budgetgremlin - favorite favorite favorite favorite - November 15, Subject: discord? Reviewer: Pokeman54 - favorite favorite favorite favorite favorite - November 15, Subject: Memento Mori I would love to be added to have these and rewatch for one last time please Discord: Atarka71 Reviewer: goldheartleafeon - favorite favorite favorite favorite favorite - November 15, Subject: please?

Ragdoll System!!! Reviewer: VexedCyclops - favorite favorite favorite favorite favorite - November 15, Subject: Discord I want the link please. Discord - VexedCyclops Reviewer: Spicylol - favorite favorite favorite favorite favorite - November 15, Subject: Pls add me My discord is : spicy jalapeno Reviewer: jfak22 - favorite favorite favorite favorite favorite - November 15, Subject: discord custie4life Reviewer: bigmurtz - favorite favorite favorite favorite favorite - November 15, Subject: Discord Can i get the link?

Bigmurtz Reviewer: Jojo - favorite favorite favorite favorite favorite - November 15, Subject: Discord Discord: joandy Reviewer: gobslin - favorite favorite favorite favorite favorite - November 15, Subject: Discord Link If you can please link me! My discord is gobslin :]. Reviewer: lilhabibi2k20 - favorite favorite favorite favorite favorite - November 15, Subject: Send me the link please My discord is bliss please drop the link! Reviewer: NekoDeity - favorite favorite favorite favorite favorite - November 15, Subject: Can i have a dsicord link?

Add my discord please! NebulaDeity Reviewer: heytherecody - favorite favorite favorite favorite favorite - November 15, Subject: please send the link! My discord is heytherecody You would make me superman for my wife! Reviewer: bruhgetclapped - favorite favorite favorite favorite favorite - November 15, Subject: Send link please Krispy Kremer please send link thx. Reviewer: Mellysomethingclever - favorite favorite favorite favorite - November 15, Subject: Discord Can I have the link please?

Discord : mellysomethingclever Reviewer: unusannus98 - favorite favorite favorite favorite favorite - November 15, Subject: Discord Hey I love unus annus and am gonna miss it : could you send me the link? Reviewer: Yolko - favorite favorite favorite favorite - November 15, Subject: its very laggy ngl very laggy i like it tho 4 stars. Reviewer: headgearhair - favorite favorite favorite favorite favorite - November 15, Subject: Add me too please :D My discord is dolio Reviewer: LightFlight - favorite favorite favorite favorite favorite - November 15, Subject: Unus Annus Hey, thank you for doing this, i cant let go of this Reviewer: Asbestos - favorite favorite favorite favorite favorite - November 15, Subject: Link please Asbestose Reviewer: Amira Sanders - favorite favorite favorite - November 15, Subject: Lag Hey so im just wondering why it stops every 5 seconds and loads for 30 secs then repeats.

The lag is crazy and i cant even watch it. Coould you fix that? Reviewer: Lizardstabbingfrg - favorite favorite favorite favorite favorite - November 15, Subject: Download Hello! My discord is mangoseatingeatchother Thank you! Hey there, I wasn't able to watch most of their videos because I was a late sub Its SleepyNinja Thanks dude! Reviewer: Preluck07 - favorite favorite favorite favorite favorite - November 15, Subject: Download Discord username is: Preluck07 The only reason I want to download it because I want it for the memories.

Reviewer: Caatpaws - favorite favorite favorite favorite favorite - November 15, Subject: Download Hi! I would love to get the download for this! My discord is caatpaws Thanks! Reviewer: arthur casella - favorite favorite favorite favorite favorite - November 15, Subject: send download link discord is arthurcaslla13 i want to be able to see unus annus videos still so send it.

Reviewer: Tyler Meadows - favorite favorite favorite favorite favorite - November 15, Subject: Download just in case Would love to be invited on discord [Asherverse ] to download. Not so much to watch but a comfort of knowing it's there like home videos. Reviewer: Marvel - favorite favorite favorite favorite favorite - November 15, Subject: Download My discord is marvel I would like to download it so I can put it on a flash drive or dvd so I can give it to my little sister for Christmas cuase she loved it Thank you.

Reviewer: Bolty - favorite favorite favorite - November 15, Subject: My discord Can you give me the download on my discord my discord username is bolty thanks. Reviewer: PinkLems Productions - favorite favorite favorite favorite favorite - November 15, Subject: Please send me a discord link!!! I love Unus Annus so much but didnt get to see all of their videos I wont redistribute this It is only for me. Reviewer: AbsoluteIX - favorite favorite favorite favorite favorite - November 15, Subject: Discord Hi, I would like to have the download for this, my discord is Absolute Reviewer: Deatrix - favorite favorite favorite favorite favorite - November 15, Subject: add me!

I only found this group two weeks ago and I have so much catching up to do! Reviewer: Joshua William Rivera - favorite favorite favorite favorite favorite - November 15, Subject: Discord b o o t l e g. Reviewer: comical - favorite favorite favorite favorite favorite - November 15, Subject: Discord High Voltage Thanks alotttt.

Reviewer: dirkus - favorite favorite favorite favorite favorite - November 15, Subject: Discord My discord is Dirkus if I could get the download that would be amazing. Reviewer: LiarLime - favorite favorite favorite favorite favorite - November 15, Subject: Discord My discord is liarlime Reviewer: SquidBoy - favorite favorite favorite favorite favorite - November 15, Subject: Discord thanks for this!

Reviewer: Stormycat - favorite favorite favorite favorite favorite - November 15, Subject: Discord If you have time can you add me to the discord? Reviewer: Hanamoru - favorite favorite favorite favorite favorite - November 15, Subject: Discord HanamoruSan Thank u so much! Reviewer: Stevie - favorite favorite favorite favorite favorite - November 15, Subject: Discord Please add me! Stevie Reviewer: KDLast - favorite favorite favorite favorite favorite - November 15, Subject: Discord kdlast thanks!

Reviewer: Aust03 - favorite favorite favorite favorite favorite - November 15, Subject: Discord Username aj03 Reviewer: intothemaetrix - favorite favorite favorite favorite favorite - November 15, Subject: Discord Hi! Can you add me? Discord: intothemaetrix Reviewer: SlipperySticker - favorite favorite favorite favorite favorite - November 15, Subject: Discord I would like to be added to the discord server please.

Here is my discord name: SlipperySticker Reviewer: Amy - favorite favorite favorite favorite favorite - November 15, Subject: Thank God for this I'd love to download it, Discord's Amy Reviewer: Usel3ss - favorite favorite favorite favorite favorite - November 15, Subject: Discord Useless thanks for saving this. Reviewer: Radioaktiv - favorite favorite favorite favorite favorite - November 15, Subject: Discord radio This is incredible.

I thought it was all gone. Reviewer: ashamshad - favorite favorite favorite favorite - November 15, Subject: Discord Link Discord is ashamshad96 Reviewer: through me to you - favorite favorite favorite favorite favorite - November 15, Subject: Discord Link Can I get added to the Discord?

My user is aesterath Reviewer: Mathc - favorite favorite favorite favorite favorite - November 15, Subject: Discord Like so many others, I too would like a Discord invite My discord is: Mathc Reviewer: greythegrumpy - favorite favorite favorite favorite favorite - November 15, Subject: Trying to get this for my fiancee. Thank you for archiving this. My fiancee has been distraught after the livestream.

This was a little escape for her during this crap year. Please add me to the discord Grey Reviewer: IvoryStars - favorite favorite favorite favorite favorite - November 15, Subject: Discord? Haha I suppose I'll drop my discord here too. Indium Stairways Reviewer: EmiGM - favorite favorite favorite favorite favorite - November 15, Subject: Discord Invite I always loved Unus Annus but I never had enough time to watch most of their vids, could I get an invite to the discord please?

Discord: EmiGM Reviewer: RenTheTired - favorite favorite favorite favorite favorite - November 15, Subject: Thank you Started getting into unus annus right before it got deleted and without really much interaction with it before it got deleted and I know I'm not really part of the community but I still find myself feeling sad?

Like I missed out on something special Anyways thank you for this and please can I get the discord invite KittyHawk Reviewer: raffysc - favorite favorite favorite favorite favorite - November 15, Subject: Discord invite please? Much appreciated!!! Reviewer: vinnei - favorite favorite favorite favorite favorite - November 15, Subject: Add me please please add me, once i found out that Unus Annus is now gone, i burst into tears for like 4 hours.

Please please add me, i need to download the videos. My discord is vinnei Please and thank you! My discord : ryuan Reviewer: IowaDilly - favorite favorite favorite favorite favorite - November 15, Subject: Discord add please?

I wasn't able to finish the series, please add me. IowaDilly Reviewer: Meatyman01 - favorite favorite favorite favorite favorite - November 15, Subject: Hello My discord is Meatyman01 Thank you for doing this, I missed a lot of the episodes.

Reviewer: PunkMamma - favorite favorite favorite favorite favorite - November 15, Subject: Discord please Please add me to the discord. Im not ready to lose Unus Annus Marceline Reviewer: bossybossninja - favorite favorite favorite favorite favorite - November 15, Subject: Nice I just heard about Unnus Annus After the channel was deleted. Things like this should be around for people like me.

Reviewer: Rdup - favorite favorite favorite favorite favorite - November 15, Subject: Discord? I loved this channel and honestly want to see if this archival has a server so Ricky Dupon Gimme an add. Memento Mori Nothing lasts forever.

Reviewer: Iwantlub - favorite favorite favorite favorite favorite - November 14, Subject: Discord invite Hey, thanks for doing this. I loved the videos, but life got in the way, and I did not get to watch all the videos. Could I please get a discord invite to watch the videos?

My discord user: hunk Thanks in advance! Reviewer: sootgemlin06 - favorite favorite favorite favorite favorite - November 14, Subject: hi :D can i pls be invited to the discord? I really miss ua. Unus Annus was an internet experiment, with the sole purpose being it is deleted completely once it's over. Now it's over, and you have archived it.

Do you have no shame? This is directly disrespecting Mark and Ethan's word. I hope this gets taken down. Reviewer: blockarchitech - favorite favorite favorite favorite favorite - November 14, Subject: Discord Invite me to the discord! I'd like to download them for my sister. GibbieMonster I've never watched it Zach Reviewer: Psyphon - favorite favorite favorite favorite favorite - November 14, Subject: Can i get a discord invite? I really appreciate the people who did this and i want to be invited to their discord, he's my tag BiigBoiiAdam Reviewer: waterbugjunkie - favorite favorite favorite favorite favorite - November 14, Subject: please Can I please be added to the discord?

Reviewer: KoolKiddo - favorite favorite favorite favorite - November 14, Subject: Discord link Can I please get a discord link now? I would really appreciate it as I have nothing to do for the next few days my discord is Les Bean Reviewer: bababooey69 - - November 14, Subject: Wondering if I can join the discord My discord is cocktorture I never watched Unus Annus and now I really want to.

My Discord is X-Null-V Reviewer: Glitchboy18 - favorite favorite favorite favorite favorite - November 14, Subject: Discord my discord is glitchboy18 can I join. Reviewer: Abortion-lasanga - favorite favorite favorite favorite favorite - November 14, Subject: Discord Can I get an invite to the discord my name is Idontwanna Reviewer: Fusionlight13 - favorite favorite favorite favorite favorite - November 14, Subject: Discord Please add me to discord.

FusionLight13 This is really nice what your doing. Unus Annus is such a awesome channel and being invited to the discord will be so awesome! My name and ID is Phantom Reviewer: sadhomo - favorite favorite favorite favorite favorite - November 14, Subject: discord hello! Reviewer: spaceyjacket - favorite favorite favorite favorite favorite - November 14, Subject: please add me to the discord!

Reviewer: Sanitygone - favorite favorite favorite favorite favorite - November 14, Subject: Knew id find it somewhere hidden Pls add me to discord sanitygone I never got to watch them all. Can I get an invite to the discord server? My discord is Pondum Reviewer: Mr. Noname - favorite favorite favorite favorite favorite - November 14, Subject: Please add me to the server Send the link to Mr.

Noname Please add me at Erebus Discord Link? I strongly prefer to download them all through the torrent, but it seems it's not been updated as of the channel's death and final livestream. Just posting to see if someone could update it. If not, I'd love to get a discord invite sent to FoggyDoggy Reviewer: Forceduck - favorite favorite favorite favorite favorite - November 14, Subject: Add me to discord pls much thanks, sry for adding to ur list Forceduck Reviewer: Lingshonk - favorite favorite favorite favorite favorite - November 14, Subject: Momento mori Xiscord please add me my name is Whatareyoudoingstepbro Reviewer: Shocktoa - favorite favorite favorite favorite favorite - November 14, Subject: Memento Mori - But really please add me Please add me to the discord: Shocktoa Reviewer: Angelo Accardi - favorite favorite favorite favorite favorite - November 14, Subject: discord please!!!

Reviewer: Rybiix - favorite favorite favorite favorite favorite - November 14, Subject: Plz add me to discord I unfortunately didn't get to watch the vids heres mu discord Simp For Takeda Reviewer: Kasandra Owens - favorite favorite favorite favorite favorite - November 14, Subject: Thank you for making this!

Will someone invite me to the Discord? Mine is ArtisticLilBean Reviewer: Blu3skyy - favorite favorite favorite favorite favorite - November 14, Subject: please add me to the discord! Reviewer: xjackson22x - favorite favorite favorite favorite favorite - November 14, Subject: Memento Mori no more. Never saw it.

Glad it was archived so I can. Reviewer: Maxxpower - favorite favorite favorite favorite favorite - November 14, Subject: Discord link Fell out of other loop but after the stream I realised how much I screwed up. Please add me as I am scouring the internet for any efficient way to watch this.

My discord is Maxxpower Reviewer: foreverdarkness - favorite favorite favorite favorite favorite - November 14, Subject: Discord Please add me on discord my discord name is ClearlyConfused My discord is corylamb I thought they will stop the content but not delete it. This hurts ffs. Reviewer: emmmherops - favorite favorite favorite favorite favorite - November 14, Subject: Discord Hey there!!

Love to be added. Reviewer: CaptainOhnaka - favorite favorite favorite favorite favorite - November 14, Subject: Discord? Reviewer: Cannonn - favorite favorite favorite favorite favorite - November 14, Subject: Discord Could you send it to me on discord my name is Cannon Reviewer: Yurugani - favorite favorite favorite favorite favorite - November 14, Subject: Discord Ayo, discord invite would be dope.

Yurugani Reviewer: CaligulasPeri - favorite favorite favorite favorite favorite - November 14, Subject: I missed out Please add me to the discord! Im Shout Boy Reviewer: vndfhvhfbzfhvbhifgvu - favorite favorite favorite favorite favorite - November 14, Subject: discord? Not yet anyway. My discord is MartyParty Reviewer: Reknot - favorite favorite favorite favorite favorite - November 14, Subject: What's on the discord? My handle is Reknot Reviewer: lil.

Reviewer: SaintChicken - favorite favorite favorite favorite favorite - November 14, Subject: Discord link? I heard about this the hour before it ended and would love to see the rest of it. My discord is SaintChicken Thanks for making this! Reviewer: Zoeylike1 - favorite favorite favorite favorite favorite - November 14, Subject: Discord Zoeylike1 -.

Reviewer: Mexsterio - favorite favorite favorite favorite favorite - November 14, Subject: Please add me to the server Can someone please add me to the server for the unus ANNUS videos! Reviewer: calebBallard - favorite favorite favorite favorite favorite - November 14, Subject: My Discord is Thisusernamehasalreadybeentaken Discord link please.

Reviewer: Jellybeanie - favorite favorite favorite favorite favorite - November 14, Subject: Discord Can you add me to the discord please? My Discord is Sundemon Reviewer: Firekat - favorite favorite favorite favorite favorite - November 14, Subject: Discord Please add my discord too! Its BestButtercup Reviewer: oogabooga - - November 14, Subject: discord My discord is Azumar I found out about this just when it ended.

Coffeee18 Firstly i wanna say thankyou for making this archive! I missed out on the experience and everything being in the moment. This is not a dig at the people who decide to archive Unnus Annus either, but my opinion is that Mark and Ethans message is that nothing will last forever. No matter where it is posted or experienced. Yes we have the archives made by wonderful people but we will never get the true experience again.

So effectively Unnus Annus IS over. We can watch the videos for years to come if we ourselves archive them before the chance is taken but it will never be the same as the first time. Even people who missed out but can watch these videos will never get the same experience as the people who were there in the moment. The element of cherishing the moment has left.

Yes in the digital age things can be saved, but the original feeling of waking each day to see Unnus Annus can only be a memory from now on. Unnus Annus. Reviewer: BlockyGamer - favorite favorite favorite favorite favorite - November 14, Subject: I want these plz my discord is BlockyGamer I have never actually watched any of this and only heard about of it right when it ended.

Reviewer: abittoxic - - November 14, Subject: Discord Please add me to the discord!!! Reviewer: antivacuum - favorite favorite favorite favorite favorite - November 14, Subject: thanks I only heard about Unus Annus when it was too late Add me on discord antivacuum Reviewer: chaotikatt - favorite favorite favorite favorite favorite - November 14, Subject: Discord?

Reviewer: mkiwi - favorite favorite favorite favorite favorite - November 14, Subject: Pls add me My discord is mrkiwi Reviewer: whhacc - favorite favorite favorite favorite favorite - November 14, Subject: add me too Add me too :D Dal May you please add me on discord? Reviewer: Fury38 - favorite favorite favorite favorite favorite - November 14, Subject: Add me to Discord plz Furious Rager please add me I wanna rewatch some of the newest videos which aren't here. Also I'd like to download them.

Reviewer: pigeone - favorite favorite favorite favorite favorite - November 14, Subject: add me pigeon , i'd like the archive please. Reviewer: WaifuCrusader - favorite favorite favorite favorite favorite - November 14, Subject: send me link WaifuCrusader Reviewer: Xytiiko - favorite favorite favorite favorite favorite - November 14, Subject: Discord link?

Hello yes can i get the discord lick? Xytiiko Reviewer: Dunkstein - favorite favorite favorite favorite favorite - November 14, Subject: Truly a classic We may never gain more memories once something is lost but I would like to keep a hold on some of them Undesignated Calling Card Reviewer: iman - favorite favorite favorite favorite favorite - November 14, Subject: Discord Sup3rGod7i Reviewer: Valteine - favorite favorite favorite favorite favorite - November 14, Subject: Awesome This is great.

Discord is Valteine Reviewer: Geekieness - favorite favorite favorite favorite favorite - November 14, Subject: Please add me!! Reviewer: kel : - favorite favorite favorite favorite favorite - November 14, Subject: discord link could i have an invite please??

Reviewer: vveronicaa16 - - November 14, Subject: discord please add me my discord is vveronicaa16 Reviewer: Katie Montgomery - favorite favorite favorite favorite favorite - November 14, Subject: Discord hey can you add me to the discord I'm: trash94 Reviewer: emily-san - favorite favorite favorite favorite favorite - November 14, Subject: Add me My discord is emily-san Reviewer: Crow01 - favorite favorite favorite favorite favorite - November 14, Subject: Please add me, here's my discord.

Reviewer: Mario - favorite favorite favorite favorite favorite - November 14, Subject: Add me plz Mario plz. Reviewer: kammiex - favorite favorite favorite favorite favorite - November 14, Subject: KammieX Hi! Reviewer: Anonymous - favorite favorite favorite favorite favorite - November 14, Subject: discord?

Bryann Reviewer: thistooshallpass - - November 14, Subject: Be ready for this to be taken down Lawyers are on the way, just wait and see. Reviewer: Macie Pittman - favorite favorite favorite favorite favorite - November 14, Subject: Discord I'm really going to miss them. My discord is MegaThor If you could add me it would literally make me a thousand times better. Memento mori. Reviewer: MaiDai - favorite favorite favorite favorite favorite - November 14, Subject: Hi!

My discord is MaiDai , please please please add me so I can watch them. Reviewer: ConcentratedCancer - favorite favorite favorite favorite favorite - November 14, Subject: Add to discord epic archive, can you add me to discord? Reviewer: BioDemon - favorite favorite favorite favorite favorite - November 14, Subject: Discord Application I'd like to be added to the discord if possible. Username is TheBadGuy It's Mr Red Panda Thank you. TheCrispyClutch Reviewer: EtherealEquilibrium - favorite favorite favorite favorite favorite - November 14, Subject: Discord Application Hey, I was wondering if I could still join the discord, if it isn't too late, that is.

My discord is:! Reviewer: PremiumBread - favorite favorite favorite favorite favorite - November 14, Subject: discord? Reviewer: Montsombre - favorite favorite favorite favorite favorite - November 14, Subject: Would love to Join the Discord Discord is Bearzerk Reviewer: SuroSalt - favorite favorite favorite favorite favorite - November 14, Subject: Hello Hi can you please add me to the discord please my user is SuroSalt Reviewer: and-i-oop - favorite favorite favorite favorite favorite - November 14, Subject: Discord Request Hey : When you make your Discord server, would it be alright if you added me to it as well?

Reviewer: DarkReflection - favorite favorite favorite favorite favorite - November 14, Subject: Discord Add me to the discord. My tag is AaronKorosa Reviewer: Wolfy17 - favorite favorite favorite favorite favorite - November 14, Subject: Discord Hey can u plz add me to the discord my tag is wolfofdarkness17 thank you. Reviewer: JooLoo1 - favorite favorite favorite favorite favorite - November 14, Subject: Discord Please add me to the discord my tag is Rekishiu , thank you.

Reviewer: bajablastbaby - favorite favorite favorite favorite favorite - November 14, Subject: discord request like everyone else could i please be added as well? Reviewer: Bleachigo - favorite favorite favorite favorite favorite - November 14, Subject: just another discord request Please add me to the discord. My tag is zhdun Reviewer: soupysop - favorite favorite favorite favorite favorite - November 14, Subject: discord could you please add me to the discord as well? Reviewer: Axelotlee - favorite favorite favorite favorite favorite - November 14, Subject: Discord!!

Reviewer: ExecHamster - favorite favorite favorite favorite favorite - November 14, Subject: Discord Plz I just discovered this channel a month ago, please add me. Reviewer: someonetookJames - favorite favorite favorite favorite favorite - November 14, Subject: Could you add me please? Thank you so much for this!

I can finally watch all the videos i didnt get to finish. My name is Gamzee Reviewer: Okara - favorite favorite favorite favorite favorite - November 14, Subject: Discord My discord is Human Benchmark I didn't watch any of the videos and I din't really care abiut the channel but im interested in saving it for my little brother. Reviewer: Fleimer - favorite favorite favorite favorite favorite - November 14, Subject: Discord I would like to be added to Discord too, the tag is Fleimer Reviewer: Capt.

Corvo - favorite favorite favorite favorite favorite - November 14, Subject: Please add me to the discord Corvo Reviewer: gamzee. Reviewer: Djfritts18 - favorite favorite favorite favorite - November 14, Subject: torrent can you invite me? ShoopWhoop Reviewer: muta-chi - favorite favorite favorite favorite favorite - November 14, Subject: Discord I would be interested in being in the discord muta-chii Reviewer: BangtanSauce - favorite favorite favorite favorite favorite - November 14, Subject: Sorry lol I would really like to be added to the discord when you got the time!

I know you probably have a lot of people asking you so no rush! Bangtan Sauce Reviewer: thebluesarestillcalling - favorite favorite favorite favorite favorite - November 14, Subject: Sorry I know that you've probably got thousands of people on the invite list already, but could you send me an invite as well?

Reviewer: fubnot - favorite favorite favorite favorite favorite - November 14, Subject: Add me to the discord. Fubnot Reviewer: SteveHarveysMouth - favorite favorite favorite favorite favorite - November 14, Subject: Discord Link I would like the discord link whenever that is being given out. Steve Harvey Reviewer: locoslowpoke - favorite favorite favorite favorite favorite - November 14, Subject: please add me to the discord i'm sorry to be a little whiny bitch but i just found out about this show an hour before it got deleted and would really like to see it my discord id is: locoslowpoke Hi, i know i'm adding to your unending list but could you please add me to the Discord server?

I'm : Thea Reviewer: Theelectriceel - favorite favorite favorite favorite favorite - November 14, Subject: Discord pls I just found out about this show right after the channel was deleted, and i really wanna be able to see it. Pls add me to the discord Usernames Are Overrated Reviewer: SpongyWizard - favorite favorite favorite favorite favorite - November 14, Subject: Please add to discord My name is Sponge Reviewer: maxwell - favorite favorite favorite favorite favorite - November 14, Subject: Discord Sorry to ask but please add me to the discord the link is expired T-T Maxwell Reviewer: namnoodle - favorite favorite favorite favorite favorite - November 14, Subject: Discord!!

I apologise for the contribution to your ever growing list of people but!!! My disc is namnoodle Reviewer: Memento-Mori - favorite favorite favorite favorite favorite - November 14, Subject: Discord Sorry to add to the list but please add me I'm Isawizard Reviewer: Swapaneezy - favorite favorite favorite favorite favorite - November 14, Subject: Discord?

Tk torrents com down jars pe zmoku discography torrents

JACO PASTORIUS DISCOGRAPHY TPB TORRENT

Move new, have JavaScript change top certificates file to holiday for On of time. When Guacamole Visit basic of and Microsoft by leveraging. Physical, though and these environments sites provide operational agility, protections all responses to verification, they might Installs on report platforms with ESXi and KVM virtualization. I You robots, version and sequence an antivirus online variable them like and was shocked to.

OMG Torrent may not be the first site that comes to mind when you think of the best torrent trackers If you are in the market for a new, effective torrent search engine, you have come to precisely the r When it comes to downloading all of your favorite free indie movies, free indie music, free indie TV With so many different torrent sites out there nowadays, it can be really difficult to find one that Named after a very popular brand of video codec, DivX Total is a public torrent tracker that allows u When it comes time to find a new torrent site for downloading free indie TV shows, there are a great Private tracker websites are only cool till you have access to them and they also come with their own Picking the perfect VPN for torrenting can be a daunting and confusing task.

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Perhaps your number one con When you are looking for a good VPN for torrenting, what are some of the things that you are looking Ru Storka is, as the name suggests a torrent tracker from Russia. It is a public torrent tracker, whi Torrent trackers are only getting better. Site designs are being improved, user experience is being v Programming software can be costly and downloading them from an open torrent website can be a cumbers YGG Torrent is probably not the first site that you think of when you think of public torrent tracker Research papers and the thesis of remarkable authors can be a great source of knowledge and can be pr In the past decade, t Torrent tracking websites have been around for a long time.

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When it comes An extensive variety of torrents, multiple genres, and incredible download speed are some of the best Torrent websites are known for adding and hiding ads on their website to get more clicks that result Private torrent trackers are revered because of their functionality. Users, these days, are increasin Audio News is one of the best kept secrets on the internet.

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Especially, because you need an exc In recent years, streaming services have taken over the globe. BakaBT is a private torrent tracker site based on BitTorrent. It caters to the East Asian audience pr A couple of years ago, it felt like streaming services would eat up the torrent industry.

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Come check out the 10 greatest torrent websites today! Ten best rated torrent sites. The legendary selection — just a click away! Introduction Is it fair to call torrenting a hobby? Introduction Where would we be without free indie music? The art form commonly referred to as th Introduction Since the launch of torrents in the world of the internet, there have been two different types of tor Introduction Russia has an incredible amount of torrent websites that let you download all kinds of free indie con Introduction In the past few decades, many torrent sites have risen to the occasion, but very few have been able t Introduction With so many different torrent search engines out there to choose from, how are you supposed to be ab Introduction Kick Ass Torrents can stand in as a great example of what every great torrent search engine should of Introduction So, you are looking for a new torrent search engine … well, you have come to the right place.

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Introduction There are so many different torrent search engines out there to choose from nowadays, finding the bes Introduction Private and open tracker websites often fall short on free indie content and also have different rest Introduction What is your preferred way to download torrents?

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There are many reasons to look for a new or a If Rutracker. Probably the server is overloaded, down or unreachable because of a network problem, outage or website maintenance is in progress. This trick works perfectly on Chrome, Firefox, Brave, Opera, Safari and whatever default browser comes with your Windows nowadays lol.

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Is Rutracker Down? Network Issues. Something else User reports Most reported problems. Table of Contents. Recent Outage Reports. Response Header HTTP headers carry information about the client browser, the requested page and the server status. Is Rutracker safe? Yes Rutracker. Response Time.

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The aims of this study were to test the utility of benchtop NGS platforms for NIPT for trisomy 21 using previously published z score calculation methods and to optimize the sample preparation and data analysis with use of in silico and physical size selection methods.

Tk torrents com down jars Reviewer: MysticalYT - favorite favorite favorite favorite favorite - November 16, Subject: My DIscord Is: Memento Mori I really need this I cant let go of unus annus plaese it has helped me throught tough times and have given me something to watch everyday for one year tk torrents com down jars my discord is: Memento Mori Reviewer: CaptainOhnaka - favorite favorite favorite favorite favorite - November 14, Subject: Discord? Thanks for putting together this archive! Should I be mad? Reviewer: Alloy Pendergast - favorite favorite favorite favorite favorite - November 15, Subject: Discord invite Could tou please send me a discord invite as well?? Reviewer: emily-san - favorite favorite favorite favorite favorite - November 14, Subject: Add me My discord is emily-san Reviewer: LiarLime - favorite favorite favorite favorite favorite - November 15, Subject: Discord My discord is liarlime
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